Whole mount in situ hybridization (WISH) of early chick embryos. NOGO-A expression localized to the neural plate (HH5), and was excluded from the notochord. Early expression was also evident within the primitive node and streak. Interestingly, NOGO-A is asymmetrically expressed within the primitive node (*). NOGO-A expression demarcated the head fold (HH6), neural tube (HH8) and brain vesicles (HH10). Expression was also present within developing somites (HH8 and HH10). HF, head fold; M, mesencephalon; NC, notochord; NP, neural plate; NT, neural tube; OV, optic vesicle; P, prosencephalon; PN, primitive node; PS, primitive streak; R, rhombencephalon; S, somite. Scale bars 250 μm.
Caltharp et al. BMC Developmental Biology 2007 7:32 doi:10.1186/1471-213X-7-32