A new method to transfect the hypoblast of the chick embryo reveals conservation of the regulation of an Otx2 enhancer between mouse and chick extraembryonic endoderm

doi:10.1186/1471-213X-7-25

BMC Developmental Biology

Volume 7

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Methodology article

A new method to transfect the hypoblast of the chick embryo reveals conservation of the regulation of an Otx2 enhancer between mouse and chick extraembryonic endoderm

Amanda Albazerchi¹^,2 , Olivier Cinquin¹^,3 and Claudio D Stern¹

¹Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT. UK

²Laboratory of Genetics, 425-G Henry Mall, University of Wisconsin, Madison, WI 53706, USA

³Deparment of Biochemistry, University of Wisconsin, Madison, WI 53706, USA

author email corresponding author email

BMC Developmental Biology 2007, 7:25doi:10.1186/1471-213X-7-25


Published:	2 April 2007

Additional files

Additional file 1:

Embryo cultured with hypoblast transfected with pCAB-Luc. This movie sequence covers 12 hours of development immediately following transfection, and shows activity of the luciferase reporter and movements of the hypoblast.

Format: MOV Size: 3.4MB Download file

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