Expression of mRAGE and sRAGE in neonatal and adult rat lung. Western blot and subsequent densitometry was performed probing for mRAGE and sRAGE in lung homogenates. Results are normalized to β-actin. (A) Neonatal mRAGE expression compared to adult mRAGE (*p < 0.05). (B) sRAGE expression in adults compared to that seen in neonates (**p < 0.01). (C) Representative Western blot showing sRAGE and mRAGE expression in the neonatal and adult lungs. Notably, a higher molecular weight band is seen in the neonatal soluble preps compared to the adult's and positive control. sRAGE seen in the adult and neonatal membrane preparations represents residual sRAGE remaining in pellet with insoluble proteins during sample preparation.
Lizotte et al. BMC Developmental Biology 2007 7:15 doi:10.1186/1471-213X-7-15