Additional File 1.

Supplementary Figure 1 – ClustalW multiple DNA sequence alignment of conceptually spliced coding sequences for the three embryonic actins with the PCR product (sjj_T04C12.6) used as the template for act-1(RNAi) dsRNA. The top chart shows that all three actins (labeled "act-1", "act-2", and "act-3") show a high percent identity to each other and to the PCR product used for RNAi (labeled "act_1dsRNA"). Very high similarity extends across an extensive portion of all three actin sequences, including several long stretches of 100% identity, indicating that all three embryonic actins are depleted by act-1(RNAi) in this study.

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Velarde et al. BMC Developmental Biology 2007 7:142   doi:10.1186/1471-213X-7-142