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Open AccessResearch article

Mitochondrial DNA heteroplasmy in ovine fetuses and sheep cloned by somatic cell nuclear transfer

Jörg P Burgstaller1* email, Pamela Schinogl1* email, Andras Dinnyes2,3 email, Mathias Müller1 email and Ralf Steinborn1 email

Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria

Szent István University, Gödöllö, 2103, Hungary

Agricultural Biotechnology Center, Genetic Reprogramming Group, Gödöllö, 2100, Hungary

author email corresponding author email* Contributed equally

BMC Developmental Biology 2007, 7:141doi:10.1186/1471-213X-7-141

Published: 21 December 2007

Additional files

Additional file 1:

Non-discriminative (ND) and discriminative (D) primer/TaqMan probe sets for detection of mtDNA heteroplasmy by ARMS-qPCR and REMS-qPCR.

Format: XLS Size: 21KB Download file

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Additional file 2:

Genetic variation in putative elements of the control region detected in mtDNA genotypes involved in this study.

Format: XLS Size: 21KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 3:

Extent of conservation of the amino acid changes found in mitochondrial genes of clone CA5. Partial amino acid sequence alignment of the mtDNA genes MT-CO1, MT-CO3 and MT-ND4L from 61 vertebrate species.

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Additional file 4:

GenBank accession numbers for partial or complete mtDNA sequences of somatic donor cells, their clonal offspring and foster mothers.

Format: XLS Size: 20KB Download file

This file can be viewed with: Microsoft Excel Viewer


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