Table 1

Top ranked 73 down-regulated genes in Ipf1/Pdx1-/- vs. Ipf1/Pdx1 +/+ e10.5 dorsal pancreatic buds

Genbank acc. No.
Gene Symbol
Gene Name
Fold-change

Extracellular matrix proteins



NM_009263
Spp1
secreted phosphoprotein 1
-9.8 U *
NM_033525
Npnt
Nephronectin
-6.1 U
NM_145584
Spon1
spondin 1, (f-spondin) extracellular matrix protein
-3.8 K **




Transport



NM_172479
Slc38a5
solute carrier family 38, member 5
-8.8 U
NM_011401
Slc2a3
solute carrier family 2 (facilitated glucose transporter), member 3
-2.6 K
NM_009593
Abcg1
ATP-binding cassette, sub-family G (WHITE), member 1
-2.3 U




Signaling



NM_018763
Chst2
carbohydrate sulfotransferase 2
-4.5 U
XM_618828
Erbb3
v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
-3.2 U *
NM_019946
Mgst1
microsomal glutathione S-transferase 1
-3.2 K *
NM_152815
Lins2
Lines homolog 2 (Drosophila)
-2.4 U
NM_025505
Blzf1
basic leucine zipper nuclear factor
-2.0 K




Transcription factors



NM_013627
Pax6
paired box gene 6
-5.8 K *
NM_144955
Nkx6-1
Nkx transcription factor related, locus 1 (Drosophila)
-4.2 U ***
NM_008686
Nfe2l1
nuclear factor, erythroid derived 2,-like 1
-2.6 U
NM_010938
Nrf1
nuclear respiratory factor 1
-2.2 U




Cell adhesion



NM_008532
Tacstd1
tumor-associated calcium signal transducer 1
-3.2 U
NM_009864
Cdh1
cadherin 1
-2.6 U




Cell cycle



NM_013928
Schip1
schwannomin interacting protein 1
-3.1 K+U
NM_007631
Ccnd1
cyclin D1
-2.6 U




DNA binding



NM_198169
Gmeb2
glucocorticoid modulatory element binding protein 2
-2.9 U
NM_175074
Hmgn3
high mobility group nucleosomal binding domain 3
-2.4 K




Kinases/Phosphatases



NM_152804
Plk2
polo-like kinase 2 (Drosophila)
-2.9 K
NM_011950
Mapk13
mitogen activated protein kinase 13
-2.5 U
NM_008981
Ptprg
protein tyrosine phosphatase, receptor type, G
-2.4 U




Apoptosis



NM_008410
Itm2b
integral membrane protein 2B
-2.3 U




Other



NM_026433
1810057C19Rik
RIKEN cDNA 1810057C19 gene
-6.3 U
NM_153457
Rtn1
reticulon 1
-6.1 U *
NM_018737
Ctps2
cytidine 5'-triphosphate synthase 2
-5.9 U
NM_007694
Chgb
chromogranin B
-5.9 K
NM_026238
Narfl
nuclear prelamin A recognition factor-like
-4.9 U
NM_145562
9130213B05Rik
RIKEN cDNA 9130213B05 gene
-4.8 U
NM_011255
Rbp4
retinol binding protein 4, plasma
-4.2 U
NM_178747
Gulo
gulonolactone (L-) oxidase
-4.2 U
NM_025429
Serpinb1a
serine (or cysteine) proteinase inhibitor, clade B, member 1a
-4.2 U
AI451390

ESTs, transcribed locus
-4.0 U
NM_133977
Trf
Transferring
-4.0 K
NM_009412
Tpd52
tumor protein D52
-3.8 U
NM_016962
Spast
Spastin
-3.5 U
NM_015784
Postn
periostin, osteoblast specific factor
-3.3 U
NM_133697
1110003E01Rik
RIKEN cDNA 1110003E01 gene
-3.2 U
NM_145599
Tmem34
transmembrane protein 34
-3.1 U
NM_025633
Metapl1
methionine aminopeptidase-like 1
-3.0 U
XM_125538
Sesn1
Sestrin 1
-3.0 K
NM_013471
Anxa4
annexin A4
-3.0 K
NM_008188
Thumpd3
THUMP domain containing 3
-2.8 U
NM_026323
Wfdc2
WAP four-disulfide core domain 2
-2.7 U
NM_033073
Krt2-7
keratin complex 2, basic, gene 7
-2.7 U
BB529246

ESTs
-2.7 K
NM_178600
Vkorc1
vitamin K epoxide reductase complex, subunit 1
-2.6 U
NM_026524
Mid1ip1
Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish))
-2.6 K+U
NM_008792
Pcsk2
proprotein convertase subtilisin/kexin type 2
-2.6 K
NM_013778
Akr1c13
aldo-keto reductase family 1, member C13
-2.6 K
NM_011464
Spint2
serine protease inhibitor, Kunitz type 2
-2.5 U
NM_197996
Tspan15
tetraspanin 15
-2.5 U
NM_022316
Smoc1
SPARC related modular calcium binding 1
-2.5 U
NM_019972
Sort1
sortilin 1
-2.4 U
NM_176860
2810457I06Rik
RIKEN cDNA 2810457I06 gene
-2.4 U
XM_133714
4921517J23Rik
RIKEN cDNA 4921517J23 gene
-2.4 K
NM_146057
Dap
death-associated protein
-2.4 K
NM_013492
Clu
clusterin
-2.3 U
NM_198654
4833432M17Rik
RIKEN cDNA 4833432M17 gene
-2.3 U
NM_138651
Cds2
CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2
-2.2 U
NM_145991
Hrpt2
hyperparathyroidism 2 homolog (human)
-2.2 U
NM_010485
Elavl1
ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1
-2.2 U
AI451897

ESTs, transcribed locus
-2.2 U
NM_029341
Capsl
calyphosine-like
-2.2 K
NR_001592
H19
H19 fetal liver mRNA
-2.1 U
XM_110660
AI427122
expressed sequence AI427122
-2.1 U
NM_025853
1700022L09Rik
RIKEN cDNA 1700022L09 gene
-2.1 U
NM_138606
Pim2
proviral integration site 2
-2.1 K
NM_031170
Krt2-8
Keratin complex 2, basic, gene 8
-2.0 U
NM_025975
Tcte1l
t-complex-associated-testis-expressed 1-like
-2.0 K
NM_007657
Cd9
CD9 antigen
-2.0 K

K = changes obtained from the 15 K microarray. U = changes obtained from the 21 K microarray. * = validated by qRT RT-PCR. ** = validated by qRT RT-PCR and in situ hybridization. *** = validated by qRT RT-PCR and immunohistochemistry.

Svensson et al. BMC Developmental Biology 2007 7:129   doi:10.1186/1471-213X-7-129