Table 1

Microarray analysis of changes in expression level of selected genes across the four cell populations isolated by flow cytometry. Bold numerals indicate B-statistic greater than zero, italicized numerals indicate expression levels two-fold or higher in P7 versus other populations, and grey numerals indicate 0.5 fold or lower expression levels in P7 versus other populations.

Gene Symbol

Accession number

Gene Name

fold change P7 V P4

B 7V4

fold change P7 V P5

B 7V5

fold change P7 V P6

B 7V6


ACVR2B

NM_001106

activin A receptor, type IIB

0.89

-5.4

1.37

-2.7

2.83

2.6

ADM

NM_001124

adrenomedullin

8.82

8.2

4.23

4.0

2.55

1.1

AFP

NM_001134

alpha-fetoprotein

1.61

1.8

2.06

1.7

1.88

0.4

BNC2

AK001099

Homo sapiens cDNA FLJ10237 fis, clone HEMBB1000438.

6.54

7.1

6.96

3.8

1.90

4.3

CALB1

NM_004929

calbindin 1, 28 kDa

9.32

3.3

6.36

5.5

2.48

4.6

CCNA2

NM_001237

cyclin A2

2.75

7.9

2.04

1.9

1.46

0.8

CCNE1

M74093

cyclin E1

1.98

7.6

1.43

0.8

1.15

-1.7

CD9

AK025016

Homo sapiens cDNA: FLJ21363 fis, clone COL02986

1.52

4.9

1.51

0.0

1.35

-3.8

CDH6

NM_004932

cadherin 6, type 2, K-cadherin (fetal kidney)

0.36

2.8

0.43

3.9

0.39

3.5

CEBPZ

NM_005760

CCAAT-box-binding transcription factor

6.15

0.7

2.68

0.6

1.32

-5.6

COL4A6

U04845

collagen, type IV, alpha 6

0.36

5.2

0.44

3.8

0.47

4.0

CRI1

NM_014335

CREBBP/EP300 inhibitory protein 1

2.35

2.5

2.39

2.3

2.83

4.4

CRYM

NM_001888

crystallin, mu

4.96

9.6

2.68

0.8

1.37

-3.5

CSPG2

NM_004385

chondroitin sulfate proteoglycan 2 (versican)

2.50

7.0

2.10

-1.1

2.85

2.1

CSPG6

NM_005445

chondroitin sulfate proteoglycan 6 (bamacan)

3.76

5.0

1.99

3.9

2.81

1.3

CTCF

NM_006565

CCCTC-binding factor (zinc finger protein)

1.61

-3.2

1.73

1.1

1.15

-3.3

DKFZP586A0522

AF113007

DKFZP586A0522 protein

5.35

5.5

4.76

2.9

3.84

4.6

DKK1

NM_012242

dickkopf homolog 1 (Xenopus laevis)

0.33

-0.6

0.36

5.0

0.43

3.6

DNMT3B

NM_006892

DNA (cytosine-5-)-methyltransferase 3 beta

3.14

4.7

2.68

0.9

1.97

1.1

DPPA4

NM_018189

developmental pluripotency associated 4

7.52

8.3

5.62

3.0

2.77

2.3

EDN1

NM_001955

endothelin 1

0.45

1.4

0.72

1.2

0.97

-6.7

EDNRB

NM_000115

endothelin receptor type B

3.05

6.9

2.27

0.3

1.30

-1.1

FBLN1

NM_006485

fibulin 1

0.37

5.0

0.43

2.9

0.60

1.1

FGFR1

AK001052

Homo sapiens cDNA FLJ10190 fis, clone HEMBA1004753.

0.67

1.2

0.65

-1.5

0.59

1.8

FGFR3

NM_000142

fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism)

0.39

2.3

0.37

3.6

0.42

3.0

FLJ10036

NM_017975

hypothetical protein FLJ10036 (zwilch)

4.38

3.5

2.57

2.3

2.62

4.0

FLJ12787

AK022849

hypothetical protein FLJ12787 (Src-associated protein SAW)

3.16

6.3

2.04

1.9

1.34

1.6

FLT1

NM_002019

fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)

4.23

2.0

3.10

3.4

1.67

3.5

FST

NM_006350

follistatin

0.22

4.9

0.31

-0.5

0.42

0.9

FZD7

NM_003507

frizzled homolog 7 (Drosophila)

3.76

2.0

1.71

-2.1

1.23

-5.5

GOLGB1

NM_004487

golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1

2.10

6.2

1.77

1.6

2.83

2.6

GPC4

NM_001448

glypican 4

2.93

6.6

2.68

3.1

1.81

2.1

GREM1

NM_013372

cysteine knot superfamily 1, BMP antagonist 1

0.57

-2.5

0.26

6.0

0.49

1.8

HAT1

NM_003642

histone acetyltransferase 1

6.02

3.9

2.35

0.7

1.17

-6.0

HELLS

AK021443

Homo sapiens cDNA FLJ11381 fis, clone HEMBA1000501.

6.87

7.3

6.63

2.1

5.24

1.9

HESX1

NM_003865

homeo box (expressed in ES cells) 1

2.30

5.5

1.25

-3.0

0.68

-0.8

HSPCA

D87666

heat shock 90 kDa protein 1, alpha

4.14

0.7

2.71

0.6

2.62

5.4

HSPCB

NM_007355

heat shock 90 kDa protein 1, beta

3.71

1.6

2.04

3.2

3.63

2.3

KDR

AF035121

kinase insert domain receptor (a type III receptor tyrosine kinase)

2.53

4.8

1.94

0.1

1.94

1.9

LHX2

NM_004789

LIM homeobox 2

0.04

6.1

0.17

0.5

0.48

1.1

LIFR

NM_002310

leukemia inhibitory factor receptor

1.91

1.2

1.71

1.8

2.38

1.1

LOC57146

NM_020422

promethin

1.93

4.4

2.35

1.1

4.23

3.2

MSX1

NM_002448

msh homeo box homolog 1 (Drosophila)

0.16

6.5

0.20

1.3

0.38

0.9

NUP54

NM_017426

nucleoporin 54 kDa

4.17

3.5

1.96

1.3

1.01

-7.3

OPTC

NM_014359

opticin

4.79

3.2

4.11

0.4

2.62

0.6

PAX6

NM_001604

paired box gene 6 (aniridia, keratitis)

0.04

3.5

0.13

0.9

0.29

2.5

PB1

NM_018165

polybromo 1

1.45

1.8

1.54

-0.4

2.23

1.7

PODXL

NM_005397

podocalyxin-like

7.31

4.9

2.22

0.6

1.38

-3.8

SAS10

NM_020368

disrupter of silencing 10

3.20

6.4

3.58

2.6

3.20

3.4

SCAMP3

NM_005698

secretory carrier membrane protein 3

0.48

2.9

0.51

1.4

0.57

2.0

SDFR1

NM_012428

stromal cell derived factor receptor 1

3.66

6.1

2.50

0.0

1.51

-0.8

SFRP1

NM_003012

secreted frizzled-related protein 1

3.97

3.3

2.99

1.7

1.85

-1.4

SLIT1

NM_003061

slit homolog 1 (Drosophila)

0.29

2.6

0.40

1.5

0.69

0.8

SMARCA2

NM_003070

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2

4.72

6.8

3.58

-0.3

2.50

1.1

SMO

NM_005631

smoothened homolog (Drosophila)

0.32

5.4

0.33

2.5

0.43

2.4

SOX1

NM_005986

SRY (sex determining region Y)-box 1

2.69

0.9

1.34

-2.7

1.70

0.6

STEAP2

AK026813

six transmembrane epithelial antigen of prostate 2

4.72

9.4

2.73

1.2

2.85

3.5

T

NM_003181

T, brachyury homolog (mouse)

1.04

-7.3

0.98

-7.3

1.01

-7.4

TERF1

NM_017489

telomeric repeat binding factor (NIMA-interacting) 1

11.31

4.0

6.50

4.8

2.04

3.1

TGFBR3

NM_003243

transforming growth factor, beta receptor III (betaglycan, 300 kDa)

0.37

8.5

0.39

2.6

0.52

1.8

VEGFC

NM_005429

vascular endothelial growth factor C

0.46

0.7

0.68

1.5

0.85

-3.7

ZFP42

NM_003422

zinc finger protein 42 (myeloid-specific retinoic acid-responsive)

0.37

5.1

0.56

2.3

0.80

-2.4


Laslett et al. BMC Developmental Biology 2007 7:12   doi:10.1186/1471-213X-7-12

Open Data