Genetic determinants of hyaloid and retinal vasculature in zebrafish

doi:10.1186/1471-213X-7-114

BMC Developmental Biology

Volume 7

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Alvarez Y
Cederlund ML
Cottell DC
Bill BR
Ekker SC
Torres-Vazquez J
Weinstein BM
Hyde DR
Vihtelic TS
Kennedy BN

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Alvarez Y
Cederlund ML
Cottell DC
Bill BR
Ekker SC
Torres-Vazquez J
Weinstein BM
Hyde DR
Vihtelic TS
Kennedy BN
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Research article

Genetic determinants of hyaloid and retinal vasculature in zebrafish

Yolanda Alvarez¹ , Maria L Cederlund¹ , David C Cottell² , Brent R Bill³ , Stephen C Ekker³ , Jesus Torres-Vazquez⁴ , Brant M Weinstein⁵ , David R Hyde⁶ , Thomas S Vihtelic⁶ and Breandan N Kennedy¹

¹UCD School of Biomolecular, and Biomedical Sciences, University College Dublin, Dublin 4, Ireland

²Electron Microscopy Laboratory, UCD Conway Institute, University College Dublin, Dublin 4, Ireland

³Center for Transposon Research. Department of Genetics, Cell Biology and Development. University of Minnesota, Minneapolis, MN 55455, USA

⁴Skirball Institute of Biomolecular Medicine. New York University School of Medicine, New York, NY 10016, USA

⁵Laboratory of Molecular Genetics, NICHD, NIH, Building 6B, Room 309, 6 Center Drive, Bethesda, MD 20892, USA

⁶Center for Zebrafish Research. Department of Biological Sciences. University of Notre Dame, Notre Dame, IN 46556, USA

author email corresponding author email

BMC Developmental Biology 2007, 7:114doi:10.1186/1471-213X-7-114


Published:	15 October 2007

Additional files

Additional File 1:

Physical interaction of Müller glia and retinal vasculature in adult zebrafish. A: Transverse view of peripheral retina in an adult Tg(gfap:EGFP) transgenic animal shows Müller cells (green) expanding through all retinal layers (blue: nuclear DAPI staining). B: Higher magnification shows Müller endfeet interposed with ganglion cell soma and contacting the retinal vessels (v). C: When the vascular layer is dissected from the inner interface of the retina (arrows) Müller endfeet remain attached to the vessels indicating a tight interaction. D: Blood vessel (green: Fli1-EGFP) overlying an adult retina seen from above with ganglion cell layer in the background (blue: DAPI nuclear staining). Müller cell endfeet (red: GFAP antibody) are observed on the entire surface of the inner retina, but especially concentrated along the retinal vessel, in direct contact with the vascular endothelium (yellow co-staining). GCL: ganglion cell layer; v: vessel; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer.

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Additional File 2:

Retinal vasculature in adult Plexin D1 mutants (obd) is characterised by a higher number of vessels and increased tortuosity. A: Left and right retinas from an obd mutant showing 9–10 main vascular branches radiating from the optic disc. Scale bar 1 mm. B: Higher magnification of an adult obd retina exhibiting extraneous vascular branches and loops that are never observed in wild types. Scale bar 200 μm. C: Plexin D1 obd mutant retina exhibiting increased vessel tortuosity. Scale bar 500 μm. OD: optic disc

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