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Open AccessHighly AccessMethodology article

Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in Xenopus

Julien Falk1 email, Jovana Drinjakovic1 email, Kin Mei Leung1 email, Asha Dwivedy1 email, Aoife G Regan1 email, Michael Piper1,2 email and Christine E Holt1 email

Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK

The Queensland Brain Institute, The University of Queensland, St Lucia, QLD, 4072, Australia

author email corresponding author email

BMC Developmental Biology 2007, 7:107doi:10.1186/1471-213X-7-107

Published: 27 September 2007

Additional files

Additional file 1:

Supplementary Figure 1. Distribution of early GFP-expressing cells in the brain and eye and stage dependency of eye-targeted electroporation.

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Additional file 2:

Supplementary Figure 2. Potential applications of electroporation to other animal models and projection systems.

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Additional file 3:

Supplementary Methods. Protocol describing the method to create, copy and modify an electroporation chamber.

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Additional file 4:

Supplementary Figure 3. Shapes of the electroporation chambers and electrodes used.

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