Methodology article
Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in Xenopus
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* Corresponding author: Christine E Holt ceh@mole.bio.cam.ac.uk
1 Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK
2 The Queensland Brain Institute, The University of Queensland, St Lucia, QLD, 4072, Australia
BMC Developmental Biology 2007, 7:107 doi:10.1186/1471-213X-7-107
Published: 27 September 2007Additional files
Additional file 1:
Supplementary Figure 1. Distribution of early GFP-expressing cells in the brain and eye and stage dependency of eye-targeted electroporation.
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Additional file 2:
Supplementary Figure 2. Potential applications of electroporation to other animal models and projection systems.
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Additional file 3:
Supplementary Methods. Protocol describing the method to create, copy and modify an electroporation chamber.
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Additional file 4:
Supplementary Figure 3. Shapes of the electroporation chambers and electrodes used.
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