Additional File 1.

Tbx3, Vax2, Msx2 expression and Phospho-Smad 1/5/8 localisation after alteration of BMP4 signaling in embryo culture by addition of exogenous Noggin or BMP4. (A, B) Post-culture embryo showing normal dorsal Tbx3 expression in the control non-treated and Noggin-treated optic cups respectively. (C, D) Post-culture embryo showing normal ventral Vax2 expression in the control non-treated and Noggin-treated optic cups respectively. (E) The boundary of Bmp4 expression (arrow) in the dorsal neural retina of an E11.5 embryo. (F) Post-culture embryo showing high levels of Phospho-Smad 1/5/8 labeling in the dorsal neural retina and in the lens. The extent of the highest level of BMP signaling in the retina is demarcated by arrow. (G) BMP4-treated contralateral eye showing a wider region of high level Phospho-Smad 1/5/8 labeling (arrow). (H, I) Another example of the extension of BMP signaling in the BMP4-treated optic cup (I) as compared with the control eye (H) in a post-culture embryo. Sections H and I are counterstained with methyl green. (J) Post-culture embryo showing normal Msx2 expression restricted to the dorsal neural retina and lens. (K) BMP4-treated contralateral eye showing widespread induction of Msx2 expression in the lens and the optic cup. Noggin or BMP4-soaked beads are indicated with asterisks (in A-K) and domains of gene expression are demarcated with arrows (in A-D). E-I show coronal sections of eyes. Abbreviations: L, lens vesicle; NR, neural retina.

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Behesti et al. BMC Developmental Biology 2006 6:62   doi:10.1186/1471-213X-6-62