Table 1

Primers used for PCR reactions. The sequences of the primers used to amplify PDE3A and β-actin mRNA in the oocyte cDNA are shown, along with the expected sizes of the PCR products. Sasseville et al. 2006.

Gene

Primer

Primer sequences

PCR product expected size (bp)

β-actin

β-actin-F

5'-ATCCTGACCCTCAAGTACCCCAT-3'

242

β-actin-R

5'-TACTCCTGCTTGCTGATCCACAT-3'

PDE3A

PDE3A-F

5'-GAACAGATGACACTGCTCAAGTT-3'

180

PDE3A-R

5'-GAGCAAGAATTGGTTTGTCCAG-3'

Sasseville et al. BMC Developmental Biology 2006 6:47   doi:10.1186/1471-213X-6-47