Table 1

Genes differentially expressed in T1 α(-/-) vs T1 α(+/+) lungs at E18.5.

Microarrays






Gene
 Fold
 p
 Acc #
 Function
 Lung expression and References
Transcription factors
Sp1
 -1.82
 0.01
 X60136
 regulation of transcription
 (+) (E1)
Sox11
 -1.64
 0.05
 AF009414
 regulation of transcription
 (+) (E2)
Signal transduction
Insulin-like growth factor I
 -1.92
 0.016
 X04480
 anti-apoptosis, organogenesis
 (+) (E3, E4)
Sp 17
 -1.82
 0.008
 Z46299
 cAMP-dependent protein kinase regulator
 (+) (E5, E6)
PKC delta
 1.50
 0.026
 X60304
 protein serine/threonine kinase activity
 (+) (E7)
Signal-induced proliferation assoc.1
 1.72
 0.011
 D11374
 regulation of cell cycle
 (+) UniGene Mm.3072
G protein gamma 3 linked
 1.90
 0.005
 AF069954
 RNA transport
 (+) UniGene Mm.345134
Pip5k2c
 2.35
 0.005
 AV303514
(+) UniGeneMm.22682
Cytoskeleton/ECM
big-h3
 -1.50
 0.006
 L19932
 cell adhesion
 (+) (E8)
dynein, cytoplasmic
 1.58
 0.007
 AF063229
 microtubule-based movement
 (+) (E9)
Ion channels/transport
Chloride channel regulator Icln
 -1.86
 0.03
 U72059
 chloride transport, regulation of cell volume
 (+) (E10)
Solute carrier family 31
 1.50
 0.002
 AI839005
 copper ion transporter
 (+) Unigene Mm.248637
Enzymes
YME1-like
 -2.33
 0.008
 AF090430
 metalloendopeptidase activity
 (+) Unigene Mm.23335
Holocytochrome c synthetase
 -2.01
 0.009
 NM_008222
 lyase
 (+) MGI:106911
Cytochrome P450, 51
 -1.81
 0.011
 AW122260

Splicing factor 3a1
 -1.69
 0.044
 AW120546
 nuclear mRNA splicing, via spliceosome
 (+) UniGene Mm.156914
Phosphodiesterase 7A
 -1.62
 0.0095
 U68171
 metal dependent phosphohydrolases
 (+) (E11)
Ring finger protein 13
 -1.62
 0.008
 AF037205
 ubiquitin-protein ligase activity
 (+) UniGene Mm.274360
Ptgs1
 -1.54
 0.008
 M34141
 prostaglandin biosynthesis
 (+) (E12)
Paraoxonase 2
 1.50
 0.02
 L48514
 arylesterase activity
 (+) (E13)
Protease serine 3
 1.53
 0.034
 AE000665
n.d.
Cytochrome P450 4v3
 1.57
 0.006
 BC026957
(+) UniGene Mm.245297
Branched chain aminotransferase 2
 1.66
 0.013
 AF031467
 amino acid biosynthesis
 (+) UniGene Mm.24210
Neuropsin
 1.74
 0.011
 D30785
 serine-type endopeptidase activity
 (+) (E14)
Catalase 1
 1.80
 0.016
 AV083603
 response to oxidative stress
 (+) (E15)
Thioether S-methyltransferase
 1.99
 0.012
 M88694
 methyl transferase activity
 (+) (E16)
Immune system
Histocompatibility 2, D1
 1.54
 0.002
 M69069
 antigen presentation
 (+) UniGene Mm.33263
Complement C1qa
 1.82
 0.004
 X58861
 complement activation
 (+) UniGene Mm.370
Complement C1q B chain
 1.82
 0.023
 M22531
 complement activation
 (+) UniGene Mm.2570
Ubiquitination/Degradation system
Proteasome (macropain) alpha 2
 -2.08
 0.046
 X70303
 ubiquitin-dependent protein catabolism
 (+) UniGene Mm.252255
Tetratricopeptide repeat gene
 -1.75
 0.029
 AJ002730
(+) (E17)
Ube2v2
 -1.58
 0.022
 NM_023585
 ubiquiting conjugating enzyme activity
 (+) UniGene Mm.235407
Transcription/Translation/Protein synthesis
Eukaryotic translation initiation fac.2A
 -2.15
 0.017
 AW061243
 regulation of protein biosynthesis
 (+) UniGene Mm.196220
FK506-binding protein (FKBP23)
 -1.82
 0.011
 AF040252
 peptidyl-prolyl isomerase
 (+) (E18)
Eukaryotic translation initiation fac.1A
 -1.72
 0.036
 AI132207
 translation factor activity
 (+) UniGene Mm.262037
Cell Adhesion/Migration
Vascular cell adhesion molecule 1
 -1.52
 0.032
 M84487
 cell-cell adhesion
 (+) (E19)
Apoptosis
Programmed cell death 4
 -1.54
 0.014
 D86344
 isomerase
 n.d.
Cell-cell interaction
Sema4b
 -1.69
 0.047
 AA266467
 cell differentiation, development
 n.d.
Ephrin B2
 -1.64
 0.011
 U30244
 development, neurogenesis, organogenesis
 (+) (E21)
Ephrin A3
 1.50
 0.006
 U92885
 cell atraction-repulsion
 (+) UniGene Mm.331159
Other
Exportin1/CRM1 homolog
 -2.00
 0.009
 AW123788
 protein-nucleus export
 (+) UniGene Mm.217547
NMDA receptor-regulated gene 1
 -1.92
 0.021
 AW260482
 angiogenesis, cell differentiation
 (+) UniGene Mm.275281
Smarca5
 -1.83
 0.05
 AA794509
 chromatin remodeling
 (+) (E22)
NP220
 -1.81
 0.008
 D83033
 RNA binding
 (+) UniGene Mm.132392
Angiomotin
 -1.78
 0.042
 AI854771
 cell migration
 (+) UniGene Mm.100068
IL13 receptor alpha 1
 -1.67
 0.02
 AA608387
 cytokine receptor activity
 (+) UniGene Mm.24208
Kidney cell derived transcript
 -1.69
 0.008
 U13371
 myoD family inhibitor domain containing
 (+) UniGene Mm.1314
Single strand DNA binding protein
 -1.69
 0.016
 AA881160
 DNA replication
 n.d.
Rnpc2
 -1.61
 0.018
 AA688834
 transcription co-activator
 (+) UniGene Mm.153895
ZAP3
 -1.61
 0.008
 AB033168
 differentiation
 (+) UniGene Mm.153183
ADP-ribosylation-like factor 6 IP2
 -1.57
 0.02
 AA763874
 GTP-binding protein
 (+) UniGene Mm.175403
Phosphatidylinositol glycan, class A
 -1.59
 0.025
 D31863
 GPI-anchor byosynthesis
 (+) UniGene Mm.3781
Quaking
 -1.56
 0.04
 U44940
 vasculogenesis, locomotory behavior
 (+) UniGene Mm.262294
WSB-1
 -1.52
 0.023
 AF033186
 signaling
 (+) UniGene Mm.307022
Sorting nexin 17
 1.51
 0.037
 AW123761
 receptor mediated endocytosis
 (+) UniGene Mm.6118
Disabled homolog 2 interacting protein
 1.60
 0.03
 AI837497
 GTPase activating protein
 (+) UniGene Mm.29629
Zinc finger protein 64
 1.73
 0.026
 U49046
 development, transcription
 (+) UniGene Mm.2095
Ribosomal protein L22
 1.77
 0.004
 AI853960
 ribosome biogenesis
 (+) UniGene Mm.259907

Student's t test ≤ 0.05, Mann-Whitney p ≤ 0.05, detection p ≤ 0.05; UniGene = Expression profile suggested by analysis of EST counts; (+) lung expression

Microarray n = 5, Fold negative = down-regulated genes; Fold positive = up-regulated genes. nd.= no detected; (E) references in supplemental material

Millien et al. BMC Developmental Biology 2006 6:35   doi:10.1186/1471-213X-6-35