Figure 3.

Lack of Phox2b⁺ cells in the intestinalmesenchyme of E10.5 Hlx knockout embryos. The figure shows Phox2b staining of sections from littermate Hlx^+/+ (panel A) and Hlx^-/- (panel B) embryos at E10.5. Staining was performed as described in Methods with the primary antibody at a titer of 1:500. Examples of Phox2b⁺ cells are indicated by blue arrowheads. Black bars, 100 μm.