Figure 5.

Localization of MATER protein in bovine oocytes and preimplantation embryos by confocal microscopy. Oocytes and embryos were incubated with anti-MATER peptide purified antibody (1:250) and revealed by green staining. Scale bar: 50 μm. (A) Oocytes at GV or NEBD stage. Lamins A/C are stained in red (middle row). Chromatin is stained in blue with Hoechst 33258 (lower row). (B) immature oocytes (IO), in vitro matured oocytes (MO), in vitro cultured zygote (1C), 2-, 4-, 8- cell embryos, morula (mo), expanded blastocysts (EB) and hatched blastocysts (HB).