Table 1

Antibodies

Experiment
Primary
Supplier
Secondary
Supplier

AdLacZ
mouse monoclonal anti-E-cadherin 1/50
BD Transduction Laboratories
horse anti-mouse Texas Red conjugated IgG 1/100
Vector

rabbit polyclonal anti-β-galactosidase 1/300
ICN
goat anti-rabbit fluorescein isothiocyanate (FITC) conjugated IgG 1/100
Vector
GeneJuice
mouse monoclonal anti-E-cadherin 1/50
BD Transduction Laboratories
horse anti-mouse FITC conjugated IgG 1/100
Vector

rabbit polyclonal anti-DsRed 1/3000
Clontech
Swine anti-rabbit tetramethylrhodamine isomer R (TRITC) conjugated IgG 1/100
DAKO
Small Intestine characterisation
Rat monoclonal anti-E-cadherin 1/100
Zymed
goat anti-rat Texas Red conjugated IgG 1/100
Vector

rat monoclonal anti-Troma-1 (cytokeratin 8) 1/50
DSHB, University of Iowa
goat anti-rat Texas Red conjugated IgG 1/100
Vector

mouse monoclonal anti-α smooth muscle actin 1/100
Sigma
horse anti-mouse FITC conjugated IgG 1/100
Vector

mouse monoclonal anti-sucrase-isomaltase 1/200
Gift (Dr Hans-Peter Hauri)
horse anti-mouse FITC conjugated IgG 1/100
Vector

mouse monoclonal anti-cdx2 1/100
BioGenex
horse anti-mouse FITC conjugated IgG 1/100
Vector

rabbit polyclonal anti-lysozyme 1/100
DAKO
goat anti-rabbit FITC conjugated IgG 1/100
Vector

rabbit polyclonal anti-chromogranin A 1/100
DAKO
goat anti-rabbit FITC conjugated IgG 1/100
Vector

goat polyclonal anti-HNF-4α 1/100
Santa Cruz
rabbit anti-goat FITC conjugated IgG 1/100
Vector

Quinlan et al. BMC Developmental Biology 2006 6:24   doi:10.1186/1471-213X-6-24