Figure 2.

Gata6 can be successfully ablated in the intestine of Gata6^loxP/loxP mice by expression of Cre from the Villin promoter. (A) The steady-state level of Gata6 mRNA was compared in intestines isolated from four control Gata6^loxP/+Villin-Cre (lanes 2–5) or experimental Gata6^loxP/loxPVillin-Cre (lanes 6–9) mice. Amplification of Pol2 (Polr2a) mRNA showed that similar levels of starting material were utilized in each reaction. (B) Immunohistochemistry showing that GATA6 was detected as an abundant nuclear protein in epithelial cells (arrows) of control intestines, but was detected at greatly reduced levels in Gata6^loxP/loxPVillin-Cre intestines.