Figure 4.

Expression of cnbA mRNA in AX2 and 01–5 cells. Total RNA was prepared from cells developed on HABP filters for 8 h, separated on 0.9% agarose/6.6% formaldehyde gels, blotted onto nylon membranes and hybridized with a DIG-labelled probe for cnbA. To the left, the sizes of the endogenous processed cnbA mRNA species in AX2 (0.7 kb) and of the RNAi product in 01–5 (1.2 kb) are indicated.