Proteomics of early zebrafish embryos

Additional File 1:

Protein identifications. 1 Unique spot identifier (ID) relating to figure 4A. 2 Molecular weight (MW) in kD and isoelectric point (pI) as apparent from the position on the 2D gel. 3 Databases which succeeded in identification are marked by "X". Only the first identification of each protein was included in the statistics. Disregarded identifications are marked by "(X)". 4 Description of protein function or name of homologous proteins. 5 Basis for the description: TIGR Description as assigned by TIGR zfin Protein/gene name assigned by zfin. Vega Protein/gene name assigned by Vega. NCBI Protein/gene name reported at NCBI REFSEQ Protein/gene name from Refseq. BLAST close homologue based on BLAST-search. Ensembl characterizes their orthologue predictions into UBRH (U)nique (B)est (R)eciprocal (H)it. MBRH one of (M)ultiple (B)est (R)eciprocal (H)its. RHS (R)eciprocal (H)it based on (S)ynteny around BRH. HS human, MM mouse, CE C. elegans. 6 MW and pI predicted from the sequence. 7 MOWSE score/threshold score (p < 0.05 of false positives for scores higher than threshold). 8 Peptides matched to identified protein/fingerprint masses searched. 9 Sequence coverage of the matched peptides. 10 (R)oot (M)ean (S)quare error in ppm of predicted versus apparent peptide mass (m/z). 11 Database. 12 Corresponding accession number of identified proteins. 13 Link to sequences and online resources (Ensembl and TIGR only).

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