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Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi

Miguel L Soares12, Seiki Haraguchi12, Maria-Elena Torres-Padilla1, Tibor Kalmar12, Lee Carpenter2, Graham Bell3, Alastair Morrison4, Christopher JA Ring4, Neil J Clarke4, David M Glover23 and Magdalena Zernicka-Goetz12*

Author Affiliations

1 The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK

2 Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK

3 Polgen Division, Cyclacel Ltd, Babraham Science Park, Babraham, Cambridge CB4 2AT, UK

4 GlaxoSmithKline Research and Development, Gunnels Wood Road, Stevenage, SG1 2NY, UK

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BMC Developmental Biology 2005, 5:28  doi:10.1186/1471-213X-5-28

Published: 28 December 2005

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Additional File 1:

Figure 1. Method for RNAi in peri-implantation development. Schematic representation of the proposed method for transient loss of gene function by electroporation followed by uterine transfer and development in utero. Approaches for the assessment of gene-specific knock-down and phenotypic analyses are suggested. Table 1. Proportion of dsBmp4 RNA-electroporated embryos with defective expression of indicated marker genes. Group 1, embryos morphologically normal (MN) and developmentally delayed or arrested (DA); group 2, morphologically defective embryos (Df). SExp, symmetrically expanded expression around the distal tip; DR, distally (DVE) restricted expression; Abs, absence of expression.

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