Additional File 3.

Complete list of gene expression patterns for all in situ hybridizations performed. Of the 323 RBPs examined, 221 showed restricted expression patterns in the brain. The remaining genes either show restricted expression in non-neural tissues, ubiquitous expression that is difficult to distinguish from background, or no expression. Caution is needed in interpreting the results. First, non-expression could be due to the sensitivity limit of non-radioactive in situ hybridization. Second, the background level of individual probes may differ. Third, some probes with high background hybridization may mask the real expression of the transcript. Fourth, we cannot rule out the possibility that some probes may show variable levels of background hybridization in different brain areas, resulting in a false positive signal. Columns A-D describe the LocusID, gene name, type of RBD, and number (internal Mahoney reference number). Columns E and, L (E13.5, P0 "Informativity"): "1" for restricted expression in the nervous system and "0" for either ubiquitous expression that is difficult to distinguish from background or no expression. As noted in Gray et al [25], some of the genes in the "0" category show uneven signals in different brain regions and are also annotated in the subsequent columns. Columns F and M (E13.5, P0 "Specificity"): "1" for restricted expression in neural tissues only, "2" for restricted expression in neural tissue with distinguishable expression in non-neural tissue, "3" for ubiquitous or no expression, and "4" for expression in non-neural tissues only. Columns G-K and N-U (E13.5, P0 "Expression"): "2" for expression, "1" for ubiquitous expression or background, "0" for no expression.

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McKee et al. BMC Developmental Biology 2005 5:14   doi:10.1186/1471-213X-5-14