Northern blot analysis of putative Wnt-1 responsive genes after retroviral infection of C57MG cells. C57MG cells were independently infected with either a Wnt-1 or empty vector retrovirus. Forty-eight hours post-infection, cells were split into media containing 2.5 μg/ml puromycin. Infected cells were passaged in the presence of puromycin for three weeks after which time total RNA was isolated from each cell line when cells were approximately 95% confluent. Northern blot analysis was performed using five μg of total RNA, and probes were prepared from the sequences obtained. Blots were subsequently probed for GAPDH. Signals were quantitated using the PhosphorImager.
Taneyhill and Pennica BMC Developmental Biology 2004 4:6 doi:10.1186/1471-213X-4-6