Dedifferentiating myofibres do not enter the cell cycle. 4 dpa regenerating tails were fixed 24 hours after intraperitoneal injection of BrdU and immunostained against BrdU (green), Mef2 (red, marking the nuclei of myoblasts and myofibres) and Dystrophin (white, marking the cell membrane of the myofibres). Dedifferentiating myofibres-the ones with irregular shapes-never showed myonuclei positive for BrdU (arrows; n = 100 myonuclei (Mef2+ nuclei inside the dystrophin)).
Rodrigues et al. BMC Developmental Biology 2012 12:9 doi:10.1186/1471-213X-12-9