Expression levels of cell adhesion proteins Cad-1 and CsaA during aggregation: The aggregates were developed on non-nutrient agar plates in the presence or absence of caffeine, adenosine or 10 mM glucose. The fully streamed aggregates were washed off with ice chilled Sorenson's buffer and subsequently cells were treated with lysis buffer. 5 μg of the protein was loaded in each well and the equal loading of checked by staining the membrane with Ponceau-S dye. 2nd lane of the blot represents the expression of Cad-1 and CsaA form the aggregate developed in the absence of compounds of the AX2 cells grown in the presence of 3 mM caffeine. The expression levels of these proteins were quantified using image J software (NIH-USA) and the changes in expressions were normalized with loading control.
Jaiswal et al. BMC Developmental Biology 2012 12:5 doi:10.1186/1471-213X-12-5