A) Effect of adenosine on smlA- cells: Adenosine prevented the breaking of the aggregation stream leading to formation of big sized slugs and fruiting bodies. B) Effect of caffeine on countin (ctnA-) null cells: With increasing concentration of caffeine, the size of the aggregate, slug, and fruiting body gets reduced. Scale bar = 200 μm. C) Western blots of Countin in the presence or absence of either caffeine or adenosine.
Jaiswal et al. BMC Developmental Biology 2012 12:5 doi:10.1186/1471-213X-12-5