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Open Access Research article

R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes)

Linyan Zhou13, Tapas Charkraborty2, Xiangguo Yu1, Limin Wu1, Gang Liu1, Sipra Mohapatra1, Deshou Wang1* and Yoshitaka Nagahama4*

Author Affiliations

1 Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), School of Life Science, Southwest University, Chongqing, 400715, P.R. China

2 Division of Molecular Environmental Endocrinology, National Institute for Basic Biology, Okazaki, 444-8585, Japan

3 Japan Society for the Promotion of Science (JSPS), 8 Ichiban-cho, Chiyoda-ku, Tokyo, 102-8472, Japan

4 Institution for Collaborative Relations, Ehime University, 3 Bunkyo-cho, Matsuyama, 790-8577, Japan

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BMC Developmental Biology 2012, 12:36  doi:10.1186/1471-213X-12-36

Published: 7 December 2012

Abstract

Background

In mammals, R-spondin (Rspo), an activator of the Wnt/β-catenin signaling pathway, has been shown to be involved in ovarian differentiation. However, the role of the Rspo/Wnt/β-catenin signaling pathway in fish gonads is still unknown.

Results

In the present study, full-length cDNAs of Rspo1, 2 and 3 were cloned from the gonads of medaka (Oryzias latipes). The deduced amino acid sequences of mRspo1-3 were shown to have a similar structural organization. Phylogenetic analysis showed that Rspo1, 2 and 3 were specifically clustered into three distinct clads. Tissue distribution revealed that three Rspo genes were abundantly expressed in the brain and ovary. Real-time PCR analysis around hatching (S33-5dah) demonstrated that three Rspo genes were specifically enhanced in female gonads from S38. In situ hybridization (ISH) analysis demonstrated that three Rspo genes were expressed in the germ cell in ovary, but not in testis. Fluorescence multi-color ISH showed that Rspo1 was expressed in both somatic cells and germ cells at 10dah. Exposure to ethinylestradiol (EE2) in XY individuals for one week dramatically enhanced the expression of three Rspo genes both at 0dah and in adulthood.

Conclusions

These results suggest that the Rspo-activating signaling pathway is involved in the ovarian differentiation and maintenance in medaka.

Keywords:
Rspo1, 2, 3; Molecular cloning; Expression profiles; Ovarian differentiation