O2 exposure required for culmination on filters. (A) Morphology of typical strain Ax3 fruiting bodies formed at 24 h at an air-water interface on filters in ambient atmosphere (21% O2). Spores exclusively comprise the sori, which are supported aerially by cellular stalks. (B) Cells were allowed to develop for 12 h to the tipped aggregate stage before elongating to slugs, in an atmosphere of 5% O2. Filters were transferred to ambient atmosphere (21% O2) for the indicated period of time before return to 5% O2 to complete development. Culmination was quantitated by counting spores, which correlated with fruiting body formation (not shown). Results are typical of 2 independent trials.
Xu et al. BMC Developmental Biology 2012 12:31 doi:10.1186/1471-213X-12-31