Loss of SFRP1 promotes the expansion of mammosphere initiating cells. Mouse mammary epithelial cells from SFRP1+/+ and SFRP1-/- (n = 4 animals/genotype) were seeded on low attachment plates and allowed to grow for 21 days. In vitro quantification of mammpospheres formed by cells as described. Bars represent mean ± SEM number of spheres formed/20,000 seeded cells from 3 separate experiments. *p < 0.05 (significantly different from SFRP1+/+ MMEC cells using student’s t-test).
Gauger et al. BMC Developmental Biology 2012 12:25 doi:10.1186/1471-213X-12-25