Expression analysis of genes altered in SFRP1−/−animals that may be involved in mammary gland branching. For real-time PCR analysis of c-Myc, TGF-β2, Wnt4, RANKL, Rspo1, and Rspo2 gene expression, total RNA was isolated from the mammary glands of 5wk virgin SFRP1+/+ and SFRP1−/− females (n = 6 animals/genotype). Real-time PCR experiments were carried out in duplicate. The results shown represent experiments performed in duplicate and normalized to the amplification of β-actin mRNA. Bars represent mean ± SEM of the fold change with respect to vehicle treated cells. *p < 0.05, **p < 0.01 (significantly different from vehicle treated cells using student’s t-test).
Gauger et al. BMC Developmental Biology 2012 12:25 doi:10.1186/1471-213X-12-25