Protein expression analysis of the Axin2 ex vivo. Mouse mammary gland sections were subjected to immunohistochemistry and stained for the Wnt target gene, Axin2 (brown chromogen). Representative ducts are shown from explant mammary gland tissues derived from SFRP1+/+ and SFRP1−/− mice cultured in control media or media containing an exogenous Wnt ligand (Wnt3a) to induce Wnt signaling: (A) SFRP1+/+ in control media, (B) SFRP1−/− in control media, (C) SFRP1+/+ in Wnt3a media, (D) SFRP1−/− in Wnt3a media. Tissue was harvested from and assessed in 6 animals/genotype.
Gauger et al. BMC Developmental Biology 2012 12:25 doi:10.1186/1471-213X-12-25