Open Access Research article

CalpB modulates border cell migration in Drosophila egg chambers

Endre Kókai1†, Ferencz S Páldy2†, Kálmán Somogyi3, Anil Chougule2, Margit Pál3, Éva Kerekes1, Péter Deák3, Péter Friedrich4, Viktor Dombrádi1* and Géza Ádám2

1 Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen H-4032, Hungary

2 Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary

3 Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary

4 Institute of Enzymology, Hungarian Academy of Sciences, Budapest, Hungary

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BMC Developmental Biology 2012, 12:20 doi:10.1186/1471-213X-12-20

Published: 24 July 2012

Additional files

Additional file 1:

Quantification of dorsal migration defects due to theCalpB505mutation and to the downregulation ofmys, ifandrheagenes. Colored bars represent the percentage of stage 10B egg chambers in which border cells displayed normal (yellow) and delayed (green) dorsal migration. Only the dorsal component of the movement was scored in all samples. The genotypes and the corresponding numbers of the egg chambers examined are indicated at the left and the right sides of the chart, respectively.

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Additional file 2:

Western blot analysis of CalpA expression in different developmental stages ofDrosophila. Extracts prepared from early (stage 2) and late (stage 17) embryos ( A), 1st ,2nd and 3rd instar larvae, pupae, as well as from adult male and female flies (B), were separated by SDS-PAGE and probed with anti-CalpA antibody. 20 ng recombinant CalpA was loaded in the control lane. The positions of the molecular mass standards are denoted. Arrows indicate the main protein bands stained with the CalpA-specific antibody. The positions of internal standards α-tubulin in panel A and α-actin in panel B are also indicated by arrows.

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Additional file 3:

Temperature dependence of therheaRNAi effect. The diagram displays the quantification of border cell migration delays caused by the silencing of the rhea gene at 25°C and 30°C. Colored bars represent the proportion of stage 10A egg chambers in which border cells migrate 0% (pink), 1-25% (purple), 26-50% (dark blue), 51-75% (light blue), 75-99% (green) and 100% (yellow) of the wild-type distance. The genotypes and the corresponding numbers of the egg chambers examined are represented at the left and the right of the chart, respectively.

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Additional file 4:

Oligonucleotide primers and conditions used for PCR amplifications.

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