Additional file 2.
Fig. S2. BMP inhibition and FGF signaling induce the expression of early neural genes. (A-B) WISH for zicr1 and sox2 of embryos injected with mRNA coding for tBR, FGF8a or tBR + FGF8a and lacZ mRNA (cyan) and collected at stage 8 (t = 6 hpf) and each subsequent hour after until stage 10.5 (t = 10 hpf) when cultured at room temperature. (C) RT-PCR of ectodermal explants dissected from uninjected embryos (UI) or embryos injected with tBR, FGF8a or tBR+FGF8a. Genes analysed are indicated on left side, treatment on top, time of collection below panel. ODC used for loading control. All images are animal pole view with dorsal to the top.
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Rogers et al. BMC Developmental Biology 2011 11:74 doi:10.1186/1471-213X-11-74