Additional file 3.
a. Schematic diagram of the alternative splice variants detected for tammar wallaby PTCH2 relative to the human PTCH2 structure. Primers spanned exons 16-22 (red bar) and 7 splice variants (including the full length transcript) were isolated. Tammar PTCH2 has two additional introns in exon 17 and 22 (blue arrow heads) and one additional exon (21-Me; red arrow). b. Table showing the relative homologies of the epitope to which the DHH antibody was raised (recombinant mouse (Rm) Dhh amino acids 199-396) to tammar wallaby DHH, SHH and IHH. Homology is significantly lower with SHH and IHH. c. Western Blot of DHH antibody a band at 43 kDa, which is the predicted size of the tammar wallaby DHH protein in its uncleaved form. Antibody cross-reactivity with SHH or IHH would create bands at 48 and 45 kDa respectively.
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O'Hara et al. BMC Developmental Biology 2011 11:72 doi:10.1186/1471-213X-11-72