MicroRNA-184 downregulates nuclear receptor corepressor 2 in mouse spermatogenesis
1 Department of Histology & Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
2 Shanghai Key Laboratory of Reproductive Medicine, Shanghai 200025, China
BMC Developmental Biology 2011, 11:64 doi:10.1186/1471-213X-11-64Published: 24 October 2011
Additional file 1:
The high transfection efficiency of cy3-labeled scramble control nucleotide in Hela cells and GC-1spg cells. Under fluorescent light, red fluorescence could be observed in Hela cells and GC-1spg cells transfected with cy3-labeled scramble nucleotide by Lipofectamine 2000, indicating a high efficiency of transfection (more than 95% cells were transfected). A, B and C: Hela cells were transfected for 5 h, 24 h and 48 h, respectively. D: GC-1spg cells were transfected for 5 h. E, F, G and H are the same visions of A, B, C and D under normal white light, respectively.
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