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Open Access Highly Accessed Research article

MicroRNA-184 downregulates nuclear receptor corepressor 2 in mouse spermatogenesis

Jingwen Wu12, Jianqiang Bao12, Li Wang12, Yanqin Hu12 and Chen Xu12*

Author Affiliations

1 Department of Histology & Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China

2 Shanghai Key Laboratory of Reproductive Medicine, Shanghai 200025, China

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BMC Developmental Biology 2011, 11:64  doi:10.1186/1471-213X-11-64

Published: 24 October 2011

Additional files

Additional file 1:

The high transfection efficiency of cy3-labeled scramble control nucleotide in Hela cells and GC-1spg cells. Under fluorescent light, red fluorescence could be observed in Hela cells and GC-1spg cells transfected with cy3-labeled scramble nucleotide by Lipofectamine 2000, indicating a high efficiency of transfection (more than 95% cells were transfected). A, B and C: Hela cells were transfected for 5 h, 24 h and 48 h, respectively. D: GC-1spg cells were transfected for 5 h. E, F, G and H are the same visions of A, B, C and D under normal white light, respectively.

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