Table 2

Applied antibodies and retrieval systems

Primary antibodies

Cat. # (clone)

Manufacturer

Dilution

Retrieval system


CK7

M7018 (OV-TL 12/30)

Dako

1:50

Retrieval solution, High pH*

CK19

M0888 (RCK108)

Dako

1:50

Retrieval solution, High pH*

HepPar1

M7158 (OCH1E5)

Dako

1:35

Retrieval solution, High pH*

EpCAM

M0804 (BER-EP4)

Dako

1:200

Retrieval solution*

Laminin

Z0097

Dako

1:500

Proteinase K, ready to use+

SOX9

HPA001758

Sigma

1:25

Retrieval solution*

AQP1

AB3065

Chemicon

1:1200

Nestin

MAB5326 (10C2)

Chemicon

1:300


*Sections were boiled in Dako retrieval solution (S3307/S1699) for 10 minutes in a microwave oven and let to cool to room temperature for 20 minutes

+Sections incubated with Dako proteinase K (S3020) for 2½ minutes at room temperature.

Utilized secondary antibodies from Dako were anti-mouse (K4000) or anti-rabbit (K4002).

Vestentoft et al. BMC Developmental Biology 2011 11:56   doi:10.1186/1471-213X-11-56

Open Data