Determination of teneurin-1 transcription start sites. BLAT alignments to the corresponding RefSeq sequences of 2 representative clones per species obtained by 5' rapid amplification of cDNA ends are shown. The sequences of the clones are given in Table 1. The human clone h1_cl_1, the mouse clone m1_cl_1 and the chicken clone c1_cl_1 start with the annotated first exon containing the ATG translation start, whereas clones h1_cl_2, m1_cl_2 and c1_cl_2 contain up to three further non-coding exons. Based on the presence of two different transcription start sites, two alternate promoters (grey shaded areas) are postulated to control the expression of the two types of transcripts. Promoter 1 resides upstream of the first coding exon, whereas Promoter 2 is located about 100-200 kb upstream depending on the species analyzed.
Beckmann et al. BMC Developmental Biology 2011 11:35 doi:10.1186/1471-213X-11-35