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Three-dimensional-reconstruction of the zebrafish heart from a consecutive series of AFOG-stained sections at 7 dpci. Several anatomical structures can be seen: the atrium (spongy appearance, light orange), the ventricle (dense muscle tissue, intense orange), the bulbus arteriosus with abundent collagen (dark blue), the atrioventricular valve (green) and the outflow tract between the ventricle and the bulbus arteriosus (green). The post-infarct zone extends from the apex of ventricle to the half-length of the ventricle wall. The outer border of the injured area is surrounded by fibrin (red), and fibrotic tissue. The middle part of the injured area contains a loose collagen network (light blue/green).

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Chablais et al. BMC Developmental Biology 2011 11:21   doi:10.1186/1471-213X-11-21