Figure 6.

Effect of the Sciara tra-2 gene on the splicing of Drosophila dsx pre-mRNA. (A) Molecular organisation of Drosophila dsx pre-mRNA showing the male and the female splicing pattern. Exons are represented in boxes and introns by dotted lines. The female-specific exon is showing in green and the male-specific exons are shown in blue. (B) RT-PCR analyses of total RNA from D. melanogaster XX pseudomales mutant for tra-2 and either not expressing (25°C) or expressing (HS) the Sciara tra-2 transgene. TDSo#2 stands for XX pseudomale of genotype yw/w; tra-2B/Df(2R)trix,tra-2-; TDSo#2/hs-GAL4,w+; TDSo#3 stands for XX pseudomale of genotype yw/w; tra-2B/Df(2R)trix,tra-2-; TDSo#3/hs-GAL4,w+; TDSo#4 stands for XX pseudomale of genotype yw/w; tra-2B/Df(2R)trix,tra-2-; TDSo#4/hs-GAL4,w+ and TDSo#5 stands for XX pseudomale of genotype yw/w; tra-2B/Df(2R)trix,tra-2-; TDSo#5/hs-GAL4,w+. (C) RT-PCR analyses of total RNA from D. melanogaster XY males mutant for tra-2 (brothers of the XX pseudomales) expressing the Sciara tra-2 transgene. TDSo#2 stands for XY male of genotype yw/Y; tra-2B/Df(2R)trix,tra-2-; TDSo#2/hs-GAL4,w+; TDSo#3 stands for XY male of genotype yw/Y; tra-2B/Df(2R)trix,tra-2-; TDSo#3/hs-GAL4,w+; TDSo#4 stands for XY male of genotype yw/Y; tra-2B/Df(2R)trix,tra-2-; TDSo#4/hs-GAL4,w+ and TDSo#5 stands for XY male of genotype yw/Y; tra-2B/Df(2R)trix,tra-2-; TDSo#5/hs-GAL4,w+. The cross to generate these XX pseudomales and XY males flies was: females yw; tra-2B/CyO,Cy; TDSo# and males w/Y; Df(2R)trix,tra-2-/CyO,Cy; hs-GAL4,w+. As a control, the splicing of the primary transcript of gene rp49 that codes for the constitutive ribosomal protein 49 [33] was monitored.

Martín et al. BMC Developmental Biology 2011 11:19   doi:10.1186/1471-213X-11-19
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