Temporal and spatial expression of granzyme G mRNA during early embryonic development. (A) RT-PCR confirmation of the temporally expressed granzyme G gene identified by DDRT-PCR in 2-cell stage embryos. Mouse embryos were collected at unfertilized oocyte (OC), one-cell (1-C), two-cell (2-C), four-cell (4-C), eight-cell (8-cell), morula (MO), and blastocyst (BL) stages. Pregnant mouse uterine (UT) GMG cell mRNA was used as a positive control. (B) Stage-specific expression of granzyme G in the extraembryonic trophoblast during early implantation (D6-D8), middle implantation (D10-14), and late implantation (D16-20). A β-actin primer set was used as an internal control. The results are representative of three experiments.
Tsai et al. BMC Developmental Biology 2010 10:88 doi:10.1186/1471-213X-10-88