Additional file 2.

Figure S1: Analysis of D-V differences for Bcd and Hb profiles in 1×-bcd embryos. (A and B) Average raw Bcd intensity profiles on the dorsal (blue) and ventral (red) sides of 24 1×-bcd embryos when measured as a function of projected distance x from the anterior (A) or contour distance c (B). In each panel, the inset shows a magnified view of the region surrounding the Hb expression boundary positions (solid arrowheads). Listed below are p values from Student's t-tests at the indicated locations in the insets: 10^-5, 10^-5, 0.0003, 0.002, and 0.02 for panel A, and 0.004, 0.0004, 0.002, 0.01 and 0.05 for panel B. (C and D) Average normalized Hb intensity profiles on the dorsal (blue) and ventral (red) sides of the same 1×-bcd embryos when measured as a function of projected distance x from the anterior (C) or contour distance c (D). See Additional file 9-Table S1 for measured values. (E-H) Bcd-Hb input/output relationship of 1×-bcd embryos analyzed in a scatter plot (E), average input-output relationship (F), profiles of Hb expression noise in response to Bcd concentration (G) and Bcd intensity noise (H). These analyses, together with those obtained in wt embryos (Fig. 3) and 3×-bcd embryos (Fig. S2), reveal a fundamentally similar Bcd-Hb relationship on both sides of the embryos. See Additional file 9- Table S1 for measured values.

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He et al. BMC Developmental Biology 2010 10:80   doi:10.1186/1471-213X-10-80