Additional file 4.
Bradykinin induces BRB breakdown. At 5 dpf, 20 double transgenic (l-fabp:DBP-EGFP;flk1:mCherry) larvae were injected with 2 uL of 20 uM bradykinin in the right eye (B, B', C and C'). 3 hours after injection, the BRB was found to be disrupted in 18 injected eyes, as indicated by the leakage of DBP-EGFP (green) (B, C) from the hyaloid vessels (red) (B'C') which showed normal morphology. No leakage was observed in any of the BSA injected contra-lateral negative control eyes (A, A').
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Xie et al. BMC Developmental Biology 2010 10:76 doi:10.1186/1471-213X-10-76