Figure 6.

PPARγ was sufficient to initiate adipocyte differentiation from hBMSCs, but could not induce fully developed adipocytes. (A) Relative expression levels of PPARγ were determined at the indicated days by real-time PCR. (B) Adipogeic differentiation revealed by oil red O staining with PPARγ knock-down by adenovirus expressing shRNA. (C) PPARγ knock-down was verified by real-time PCR (n = 3, *P < 0.05). (D) PPARγ over-expression in hMBSCs with adenovirus (Lac Z as control) was shown by Western Blotting. (E) HBMSCs were cultured to confluence and infected with adenovirus at MOI 10 alone or in combination with indomethacin. Lipid droplets indicated by oil red O staining on day 14 (magnification 100×). (F) The expression of adipocyte marker FABP4 was detected on day six by Western Blotting. (G) Morphology of lipid droplets induced by PPARγ expression and hormone treatment (magnification 200×). (H) GLUT4 expression normalized by FABP4 was quantified by real-time PCR in cells treated with PPARγ adenovirus or hormone (n = 3, *P < 0.05).

Qian et al. BMC Developmental Biology 2010 10:47   doi:10.1186/1471-213X-10-47
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