PPARγ was sufficient to initiate adipocyte differentiation from hBMSCs, but could not induce fully developed adipocytes. (A) Relative expression levels of PPARγ were determined at the indicated days by real-time PCR. (B) Adipogeic differentiation revealed by oil red O staining with PPARγ knock-down by adenovirus expressing shRNA. (C) PPARγ knock-down was verified by real-time PCR (n = 3, *P < 0.05). (D) PPARγ over-expression in hMBSCs with adenovirus (Lac Z as control) was shown by Western Blotting. (E) HBMSCs were cultured to confluence and infected with adenovirus at MOI 10 alone or in combination with indomethacin. Lipid droplets indicated by oil red O staining on day 14 (magnification 100×). (F) The expression of adipocyte marker FABP4 was detected on day six by Western Blotting. (G) Morphology of lipid droplets induced by PPARγ expression and hormone treatment (magnification 200×). (H) GLUT4 expression normalized by FABP4 was quantified by real-time PCR in cells treated with PPARγ adenovirus or hormone (n = 3, *P < 0.05).
Qian et al. BMC Developmental Biology 2010 10:47 doi:10.1186/1471-213X-10-47