Isolation and adipogenic differentiation of hBMSCs. (A) The morphology of adherent hBMSCs three and 12 days after plating (magnification 100×). (B) HBMSCs of P5 were cultured for one week after confluence and induced to differentiate with MDI+Indo (M: methyl-isobutyl-xanthine; D: dexamethasone; I: insulin; Indo: indomethacin) treatment for one, two or three cycles (1 cycle of treatment: MDI+Indo for three days followed by insulin for one day). The accumulation of cytoplasmic triglyceride was detected by Oil Red O staining on day 21 and visualized under a microscope (magnification 100×). (C) FABP4 expression was examined by Western Blotting at the indicated days after differentiation with repeated MDI+Indo treatment (three times).
Qian et al. BMC Developmental Biology 2010 10:47 doi:10.1186/1471-213X-10-47