Limb and axial skeleton phenotypes of the 7t mutant. Panels on the left are wild type, and panels on the right are mutants. (A) Digits I-V are anterior to posterior, respectively. (B) Representative example of a Plzf7t/7t hindlimb in which digit III has been duplicated (asterisk). (C) Wild-type has one calcaneus. (D) The tatus of the Plzf7t/7t is homeotically transformed into a calcaneus. The blue triangle indicates a metatarsal fusion of digits I and II. In contrast to the wild-type hindlimbs (E), the mutants (F) display a thinner tibia that appears to be homeotically transformed into a thicker fibula-like structure. The green arrow indicates a failure of the two bones to fuse. (G, H) A dorsal view shows that the total number of thoracic vertebrae are the same in both genotypes. However, whereas the wild type has seven ribs attached to the sternum (I), Plzf7t/7tmutants have up to 10 (J). "Clav" = clavicle.
Ching et al. BMC Developmental Biology 2010 10:33 doi:10.1186/1471-213X-10-33