EDL myofibres from growing and adult mice. Entire EDL muscles from P3, P7 (a), P14, P21 and P56 (b) Myf5nlacZ/+ mice were cryosectioned and mid-belly sections stained with haematoxylin and eosin to determine total myofibre content and myofibre cross-sectional area. EDL myofibres were isolated from 3F-nlacZ-E mice at P3 (c), P7 (d), P14 (e), P21 (f) and P56 (g), fixed and incubated in X-gal solution to reveal the β-galactosidase activity of myonuclei by the presence of the blue reaction product. Images of representative myofibres were all taken at the same magnification and show that there is a 4.5-fold increase in length between P3 and adulthood (P56). Myonuclei appear to be uniformly distributed along the length of a myofibre at each age examined (c-g). Scale bar equals 100 μm for (a and b) and 1000 μm for (c-g).
White et al. BMC Developmental Biology 2010 10:21 doi:10.1186/1471-213X-10-21