Rosa-rtTA-M2 integrated into a putative gene on chromosome 2. A. Rosa-rtTA-M2 provirus inserted into intron 11 of putative gene (GeneID: 310880) on chromosome 2. E: Exon. B. Genomic PCR using primers from Exon 10 (P1) and vector (P2) verified this integration. Lane M: 1 Kb DNA ladder; lane 2, no digestion; lane 3, EcoRI digestion; lane 4, Spe I digestion; lane 5, wide type rat DNA). C. Detection of the putative endogenous gene expression in rat tissues by RT-PCR. Reverse transcription was performed with an oligo (dT)17 primer. The endogenous transcript was amplified using primers from exon 10 (P1) and exon 13 (P3) of this putative gene. M2: ROSA-rtTA-M2 rat; wt: wild type rat.
Sheng et al. BMC Developmental Biology 2010 10:17 doi:10.1186/1471-213X-10-17