RT-PCR expression pattern analysis during the early stages of embryogenesis. For each of the genes analyzed by RT-PCR the expression pattern in syncitial-stage embryos is reported together with control RT-PCR amplications on cDNA derived from unfertilized eggs and adult heads. The same primers used in RT-PCR were also used in PCR amplifications on genomic DNA. A) Expression patterns of the three known C. capitata sex determination genes: Cctra2, Cctra and Ccdsx. For the Cctra gene, the 1.1 kb splicing form is the expected male-specific mRNA, while the 0.7 kb is the female-specific form. For the Ccdsx gene the 579 bp splicing form is the expected female-specific mRNA, while the 327 bp is the male-specific form. Absence of amplification of Ccdsx from adult male genomic DNA is probably due to the presence of a long intron in the male-specific amplicon (see Figure 1). B) Expression patterns of medfly genes that share similarities to known D. melanogaster sex determination genes. C) Expression patterns of medfly genes that share similarities to known D. melanogaster cellular blastoderm formation genes. D) Expression pattern of Cczelda gene, similar to the D. melanogaster key regulatory gene of the MTZ transition event.
Gabrieli et al. BMC Developmental Biology 2010 10:12 doi:10.1186/1471-213X-10-12