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Sex and the single embryo: early deveopment in the Mediterranean fruit fly, Ceratitis capitata

Paolo Gabrieli1, Andrea Falaguerra1, Paolo Siciliano1, Ludvik M Gomulski1, Francesca Scolari1, Antigone Zacharopoulou23, Gerald Franz2, Anna R Malacrida1 and Giuliano Gasperi1*

Author Affiliations

1 Department of Animal Biology, University of Pavia, Piazza Botta 9, 27100 Pavia, Italy

2 Entomology Unit, FAO/IAEA Agriculture and Biotechnology Laboratory, Joint FAO/IAEA Programme, International Atomic Energy Agency, Vienna, Austria

3 Division of Genetics, Cell and Developmental Biology, Department of Biology, University of Patras, 26500 Patras, Greece

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BMC Developmental Biology 2010, 10:12  doi:10.1186/1471-213X-10-12

Published: 26 January 2010

Additional files

Additional file 1:

A new MITE in Ceratitis capitata. A) Southern blot analysis of male and female ISPRA genomic DNA (SspI digested) using the indel derived from the Y727 bp sequence as probe. The multiple signals are easily visible in both male and female DNA, even if the hybridization pattern is clearly different. B) Putative secondary structure of the MITE-RNA (in silico analysis using RNA-fold web server).

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Additional file 2:

Alignment of the Y-derived sequence of the putative new MITE, and two sequences from the medfly EST database. Grey boxes indicate nucleotide identities between the three sequences; red and light-blue boxes highlight the positions of the putative ITR (inverted terminal repeats), and the green boxes highlight the direct duplication of the genomic sequence of one of the three sequences.

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