Cell autonomous Shh-activated roof plate expansion causes Wnt3a expansion into choroid plexus domain. RNA in situ hybridization analysis of E12.5 wild type littermate control (A-C) and activated Smoothened expressed in the roof plate through GDF7cre regulation (D-F). Wnt3a: A, D, rTTR: B, E, Lhx5: C, F. n = 3. Arrows point to Wnt3a expansion (D) and rTTR expansion (E). Scale bar in A represents 200 μm and all panels in this figure are photographed at the same magnification (5×).
Himmelstein et al. BMC Developmental Biology 2010 10:118 doi:10.1186/1471-213X-10-118