Additional file 3.
Figure S3: Deletion of dermal Itβ1 does not disrupt skin differentiation, BMZ components or endothelial structures. Immunofluoresence for various markers in skin of control and conditional knockout mice. A: Loricrin (green) and Itα6 (red) indicate granule and corneal cell layers and basal cell layers, respectively, of interfollicular epidermis. B: Keratin 10 (green) indicates the prickle and granule cell layers of interfollicular epidermis. C-D: IF for tenascin C (red) indicates a normal lack of this marker in early dermal papilla and presence in the rest of the dermis. D: IF for fibronectin (green) indicates a normal lack of this marker in dermal papilla and presence in ECM in non-DP dermis. E: IF for perlecan (red) indicates normal endothelial structures. F-G: No differences are seen in collagen I (F, green) and collagen IV (G, green) expression in skin of control and conditional knockouts.
Format: JPEG Size: 3.7MB Download file
DeRouen et al. BMC Developmental Biology 2010 10:112 doi:10.1186/1471-213X-10-112